Skip to main content
. Author manuscript; available in PMC: 2021 Dec 29.
Published in final edited form as: Nat Neurosci. 2021 Mar 29;24(5):685–693. doi: 10.1038/s41593-021-00819-3

Fig. 3 |. Identification of inhibitory projections to the ADn.

Fig. 3 |

a,b, WT mice were micro-infused with CTB (n = 3) in the ADn, and retrograde-labeled neurons in CA3 were visualized. Sections were costained with the inhibitory cell marker GAD67. Numerous CTB+ and GAD67+ colocalized cells were visualized in CA3 (white arrows). c,d, To identify inhibitory projections to the ADn, VGAT-Cre mice were micro-infused with AAV-DIO-EYFP in CA3 (n = 3). Strong terminal projections were visualized in the ADn. e–g, To visualize specifically CA3–ADn projections and examine whether they are inhibitory, WT mice were micro-infused with AAV-DIO-mCherry bilaterally in CA3 and AAVrg-Cre-EGFP unilaterally in ADn, and sections were costained with (f) GAD67 (CA3) or (g) VGAT (ADn) (n = 4). Numerous inhibitory CA3–ADn projection neurons in CA3 (mCherry+ and GAD67+, white arrows) and projections in ADn (mCherry+ and VGAT+, white arrows) were visualized (blue scale bars, 100 μm; white scale bars, 50 μm).