Figure 2.

Data quality control and peak detection for ATAC-seq. (A) The insert size distribution of sequenced fragments had clear periodicity of ~200 bp. (B) Peak consistency between two replicates of each group indicated by IDR. x-Sample 1 log10 peak scores vs. y-Sample 2 log10 peak scores. Sample 1 and 2 are the two replicates of given group. Peaks that did not pass the IDR threshold (0.05) were colored red (https://screen.encodeproject.org/). The self-consistency ratio and rescue ratio of the adult group were 1.20 and 1.82, respectively, and as for the embryo group, they were 1.41 and 2.62, respectively. (C) TSS enrichment of ATAC-seq reads. Adult: C-6-2, D-1-1. Embryo: T-6-2, T-7-1.