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. 2021 Dec 15;8:782409. doi: 10.3389/fvets.2021.782409

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Copyright © 2021 Cao, Cheng, Huang, Lan, Lei and Chen.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Motif enrichment analysis and validation of 15 putative enhancers. (A) Enriched motifs of the 4,319 putative enhancers with AME (Supplementary Table 5). (B) Detailed information of the 15 selected putative enhancers. E, embryo-specific; A, adult-specific; log2FC, log₂ fold change (E/A); (C) Twelve putative enhancers cloned into pGL3-Promoter (SV40 Promoter/Enhancer^+/−) significantly triggered luciferase activity in cattle myoblast (P < 0.05, n = 5, compared with blank pGL3-Promoter, the raw data and significance were listed in Supplementary Table 6). HEK293T, human embryonic kidney cell line; C2C12 mouse myoblast cell line; 3T3-L1, mouse immortalized preadipocyte; MAC-T, bovine mammary alveolar cell line; myoblast, the isolated myoblasts from Qinchuan embryo muscle.