Table 1. List of primer sequences used in multiplex PCR for the determination of PMQR determinants.

Target gene	Primer sequences	Amplicon size (bp)	Amplification (30 cycles)			References
			Denaturation	Annealing	Extension
qnrA F	CAGCAAGAGGATTTCTCACG	630	94°C for 30 seconds	63°C for 90 seconds	72°C for 90 seconds	Ciesielczuk et al., 2013 [42]
qnrA R	AATCCGGCAGCACTATTACTC
qnrD F	CGAGATCAATTTACGGGGAATA	581
qnrD R	AACAAGCTGAAGCGCCTG
qnrB F	GGCTGTCAGTTCTATGATCG	488
qnrB R	GAGCAACGATGCCTGGTAG
qnrS F	GCAAGTTCATTGAACAGGGT	428
qnrS R	TCTAAACCGTCGAGTTCGGCG
oqxAB F	CCGCACCGATAAATTAGTCC	313
oqxAB R	GGCGAGGTTTTGATAGTGGA
aac(6’)-lb-cr F	TTGGAAGCGGGGACGGAM	260
aac(6’)-lb-cr R	ACACGGCTGGACCATA
qepA F	GCAGGTCCAGCAGCGGGTAG	218
qepA R	CTTCCTGCCCGAGTATCGTG
qnrC F	GCAGAATTCAGGGGTGTGAT	118
qnrC R	AACTGCTCCAAAAGCTGCTC