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. 2021 Dec 29;10:e71981. doi: 10.7554/eLife.71981

Key resources table.

Reagent type (species) or resource Designation Source or reference Identifiers Additional information
Genetic reagent (Mus musculus) Slc6a3tm1(cre)Xz/J Provided by the Xiaoxi Zhuang lab (The University of Chicago), PMID:15763133 RRID:IMSR_JAX:020080 Also available at Jackson Laboratories: https://www.jax.org/strain/020080
Chemical compound, drug 3,3’-Diaminobenzidine Sigma-Aldrich D12384 50 mg/ml
Chemical compound, drug Sodium cacodylate buffer, pH 7.4 Electron Microscopy Sciences 11653 0.2 M stock, use at 0.1 M
Chemical compound, drug Sodium hydrosulfite Sigma-Aldrich 157953 0.8% (w/v)
Chemical compound, drug Sodium bicarbonate Sigma-Aldrich S5761 0.1 M stock, used at 60% (v/v)
Chemical compound, drug Sodium carbonate Sigma-Aldrich S7795 0.1 M stock, used at 40% (v/v)
Chemical compound, drug Osimum tetroxide Electron Microscopy Sciences 19150 4% aqueous stock solution, use at 2%
Chemical compound, drug Paraformaldehyde Electron Microscopy Sciences 15710 16% aqueous stock solution, use at 2%
Chemical compound, drug Glutaraldehyde Electron Microscopy Sciences 16220 25% aqueous stock solution, use at 2.5%
Chemical compound, drug Postassium ferrocyanide Sigma-Aldrich P3289 2.5%
Chemical compound, drug Pyrogallol Sigma-Aldrich P0381 4%
Chemical compound, drug Uranyl acetate Electron Microscopy Sciences 22400–4 4% aqueous stock solution, use at 1%
Chemical compound, drug Lead (II) nitrate Sigma-Aldrich 228621 0.66%
Chemical compound, drug Embed 812 kit Electron Microscopy Sciences 14120 49% Embed 812, 28% DDSA, 21% NMA, and 2.0% DMP 30
Chemical compound, drug Cocaine Obtained through DEA license (Xiaoxi Zhuang, The University of Chicago) 10 mg/kg