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. 2021 Nov 15;10:e68584. doi: 10.7554/eLife.68584

Figure 3. The tiny limb staged regenerate has increased relative nerve abundance.

(A) Fluorescent images were obtained of transverse sections of uninjured, early, and late tiny limb stages, and fully regenerated limbs (DAPI = blue, phalloidin (for actin filaments) = red, acetylated tubulin (for nerves) = green; scale bars are 1000 µm). (B) Nerve area relative to total limb area was quantified from the sections represented in A (n = 5). Error bars = SEM. p-Values calculated by ANOVA and the Tukey post hoc test. * = p < 0.05 ** = p < 0.005.

Figure 3.

Figure 3—figure supplement 1. Absolute abundance of nerves in different staged regenerates.

Figure 3—figure supplement 1.

Total nerve area was quantified from transvers limb sections of unamputated limbs, early tiny limbs, late tiny limbs, and fully regenerated limbs (n = 5). Error bars = SEM. p-Values calculated by ANOVA and the Tukey post hoc test. * = p < 0.05 ** = p < 0.005.