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. 2021 Nov 15;10:e68584. doi: 10.7554/eLife.68584

Key resources table.

Reagent type (species) or resource Designation Source or reference Identifiers Additional information
Genetic reagent (Ambystoma mexicanum) White-strain 518 Ambystoma Genetic Stock Center at the University of Kentucky RRID: AGSC_101J
Genetic reagent (Ambystoma mexicanum) GFP-strain Ambystoma Genetic Stock Center at the University of Kentucky RRID: AGSC_110J
Genetic reagent (Ambystoma mexicanum) RFP-strain Ambystoma Genetic Stock Center at the University of Kentucky RRID: AGSC_112J
Chemical compound, drug MS-222 Sigma-Aldrich E10521-50G
Chemical compound, drug Formaldehyde RICCA Chemical Company R3190000-1A
Other DAPI Sigma-Aldrich D9542-5MG (1:1000) dilution
Chemical compound, drug Ethylenediaminetetraacetic acid (EDTA) VWR VWRV0105-500G
Commercial assay or kit In Situ Cell Death Detection Kit, Fluorescein Roche 11684795910
Commercial assay or kit Click-iT Plus EdU Proliferation Kit Roche C10337 100 ng EdU/Animal
Software, algorithm ZenPro software Zeiss 410138-1104-260
Software, algorithm FIJI ImageJ Open Source
Other Tissue-Tek OCT Compound Sakura 4583
Other Permount Mounting Medium Thermo Fisher Scientific SP15-100
Other VECTASHIELD612 Antifade Mounting Medium Vector Laboratories H-1000–10
Other Wheat-Germ Agglutinin Thermo FisherScientific W32466
Other Rhodamine phalloidin Thermo Fisher Scientific R415
Other Hematoxylin Solution, Harris Modified Sigma-Aldrich HHS16-500ml
Other Eosin Y Thermo Fisher Scientific E511-100
Other Alcian Blue Sigma-Aldrich A-5268
Antibody (Mouse Monoclonal) Anti-Acetylated Tubulin antibody Sigma-Aldrich T7451-200UL (1:200) dilution
Antibody (Goat polyclonal) Anti-Mouse IgG Alexa Fluor 488 Abcam ab150173 (1:200) dilution