Figure 5.

Evaluation of gentamicin release and its antibacterial properties. Release of gentamicin encapsulated in layer-by-layer coated titanium surfaces at 3 loading levels: 5 µg (loading level 1), 10 µg (loading level 2), and 75 µg (loading level 3). Data are presented as mean ± SD (n = 5). Data were evaluated and plotted as (A) cumulative and (B) total released amount. Analysis of the ability of gentamicin-loaded surfaces to inhibit the growth of (C) Escherichia coli and (D) Aggregatibacter actinomycetemcomitans as determined by measurement of absorbance at 600 and 620 nm, respectively. (E) Estimated half-maximal effective concentration (EC₅₀) values for free and nanolayer-encapsulated gentamicin. **P < 0.01, 1-way analysis of variance.