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(a) Molecular structures of three Raman reporters. (b) Average SERS spectra of 0.1 mM 4-MBA, 2 μM Cy3, 2 μM RRX and their 1:1:1 molar mixtures on Au nanopopcorn substrate. Characteristic Raman peaks of Cy3 at 1470 cm− 1) and RRX at 1650 cm− 1 were used for the quantitative evaluation of SARS-CoV-2 and influenza A/H1N1, respectively. The Raman peak of 4-MBA at 1075 cm− 1 was used as an internal standard.
