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(a) Absorption spectrum of complex 2 in the absence (a) and presence of 5 µM (b), 10 µM (c), 15 µM (d), 20 µM (e), 25 µM (f), 30 µM (g), 35 µM (h) and 40 µM (i) DNA. The arrow direction indicates increasing concentrations of DNA. (b) Plot of A/(A-A) vs. 1/[DNA] for binding constant determination of the complex 2-DNA adduct.
