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. Author manuscript; available in PMC: 2022 Jul 12.
Published in final edited form as: Nat Cell Biol. 2021 Jul 12;23(7):684–691. doi: 10.1038/s41556-021-00709-7

Fig. 5 ∣. Depletion of ALKBH7 in mouse tissues led to altered levels of mature mt-mRNAs and tRNAs.

Fig. 5 ∣

a, Relative mRNA levels of 13 mt-mRNAs (normalized to mouse Actb) in BAT isolated from Alkbh7-knockout mice (Alkbh7−/−) versus wild-type mice. P = 0.0002, 0.0137, 0.0373, 0.2364, 0.0005, 0.0005, 0.0097, 0.0024, 0.0102, 0.6519, 0.0567, 0.0024 and 0.6445, respectively; unpaired, two-tailed t-test. b, Relative mRNA levels of 13 mt-mRNAs (normalized to mouse Actb) in liver isolated from Alkbh7-knockout mice (Alkbh7−/−) versus wild-type mice. P = 0.0016, 0.0116, 0.0123, 0.0085, 0.0141, 0.0108, 0.0059, 0.0045, 0.0143, 0.0099, 0.0037, 0.0047 and 0.0046, respectively; unpaired, two-tailed t-test. c, Relative tRNA levels of mt-Leu1 (normalized to mouse U6 snRNA) in BAT, liver and kidney isolated from Alkbh7-knockout mice (Alkbh7−/− versus wild-type mice. P = <0.0001, 0.0221 and <0.0001, respectively; unpaired, two-tailed t-test. d, Relative tRNA levels of mt-Ile (normalized to mouse U6 snRNA) in BAT, liver and kidney isolated from Alkbh7-knockout mice (Alkbh7−/− versus wild-type mice. P = 0.0001, 0.0485 and 0.0056, respectively; unpaired, two-tailed t-test. e, Relative tRNA levels of mt-Trp (normalized to mouse U6 snRNA) in BAT, liver and kidney isolated from Alkbh7-knockout mice (Alkbh7−/−) versus wild-type mice. P = 0.4192, 0.0086 and <0.0001, respectively; unpaired, two-tailed t-test. For a–e, n = 6 biologically independent samples; data are presented as mean values ± s.d. NS, P ≥ 0.05; *P < 0.05; **P <0.01; ***P < 0.001; and ****P < 0.0001. f, Western blotting showed that Alkbh7 knockout led to decreased levels of OXPHOS complex I and III in BAT (normalized to Tomm20). Two rounds of immunoblots for biologically independent samples were performed, with similar results obtained.