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. 2021 Dec 22;2021:4525988. doi: 10.1155/2021/4525988

Figure 7.

Figure 7

VX765 treatment attenuated I/R-induced endothelial pyroptosis. (a–h) Mouse lung tissue and serum were collected from each group for the following experiments. (a–c) Caspase-1, GSDMD, and IL-1β mRNA levels measured by RT-qPCR. (d) Protein levels of pro-GSDMD, GSDMD-N, pro-caspase-1, and caspase-1 P20 detected by Western blotting. (e, f) IL-1β protein level in lung tissue and serum measured by ELISA. (g, h) Colabeled CD31 (green) with caspase-1 or GSDMD (red). DAPI was used to stain the cell nuclei by immunofluorescence; vascular endothelium (yellow arrow), scale bar, 200 μm. The data are expressed as mean ± SEM (n = 7 per group). VX765 = 30 mg/kg. ∗P < 0.05 vs. control. #P < 0.05 vs. I/R.