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. 2021 Dec 22;2021:4525988. doi: 10.1155/2021/4525988

Figure 8.

Figure 8

Effects of VX765 on PMVEC pyroptosis. (a–h) PMVECs and supernatant were collected from the controls and OGD/R group for the following experiments. (a–c) Caspase-1, GSDMD, and IL-1β mRNA levels. (d) Protein levels of pro-GSDMD, GSDMD-N, pro-caspase-1, and caspase-1 P20 measured by WB. (e) The protein level of IL-1β in the cell supernatant. (f) LDH release rate. (g, h) Immunofluorescence staining for caspase-1 or GSDMD (red). DAPI was used to stain the cell nuclei. Scale bar, 200 μm. The data are expressed as mean ± SEM. Each experiment was performed independently at least 3 times. VX765 = 10 μΜ. ∗P < 0.05 vs. control. #P < 0.05 vs. OGD/R.