. Author manuscript; available in PMC: 2021 Dec 30.

Published in final edited form as: J Comp Neurol. 2016 Feb 18;524(10):2036–2058. doi: 10.1002/cne.23930

TABLE 1.

Stereological Parameters Used for Estimating Cell Numbers in the River Hippopotamus

Nucleus examined	Counting frame size (μm)	Sampling grid size (μm)	Disector height (μm)	Cut thickness (μm)	Average mounted thickness (μm)	Vertical guard zones (top and bottom, μm)	Section interval	No. of sections	No. of sampling sites	Average CE (Gundersen m = 0)	Average CE (Gundersen m = 1)
LDT (ChAT+)	200 × 200	200 × 200	15	50	36.3	5	11	21	2,680	0.04	0.03
PPT (ChAT+)	200 × 200	800 × 800	15	50	39.4	5	11	21	484	0.07	0.06
LC (TH+)	150 × 150	800 × 800	15	50	34.4	5	11	20	440	0.10	0.11
Orexin (OxA+)	200 × 200	800 × 800	15	50	26.3	5	11	25	463	0.10	0.10

Abbreviations: LDT, laterodorsal tegmental nucleus, PPT - pedunculopontine tegmental nucleus; LC, locus coeruleus complex; ChAT+, neurons immunopositive for choline acetyltransferase; TH+, neurons immunopositive for tyrosine hydroxylase; OxA+, neurons immunopositive for orexin-A.