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Flow cytometry and quantitative real-time PCR (qRT-PCR) detection. (A) The proportion change of Th1, Th2, and Th17 cells in patients with HF (n = 10) and controls (n = 10) detected using flow cytometry. ***p < 0.001. (B) Expression changes of the key genes in HF dogs and controls through qRT-PCR detection. *p < 0.05, **p < 0.01, ***p < 0.001. qRT-PCR, quantitative real-time PCR; HF, heart failure.
