TABLE 1.
Near-infrared-I/II fluorophores related to CRISPR-Cas9.
| NIR-I/II fluorophore | Nanoparticles structures | Excitation (nm) | Emission (nm) | Character | Targets | Application | Ref |
|---|---|---|---|---|---|---|---|
| SPPF-Dex nps |
|
808 | 1,000–1,400 | Photothermal conversion, NIR-II imaging, deep tissues | MTH1, HCT 116 cells | CRISPR-Cas9 delivery, real time imaging, animal model | Li et al. (2019) |
| Au nanorod (APC) |
|
1,064 | 1,000–1,700 | Photothermal activation, deep tissues | AAVS1, asialoglycoprotein receptors targeting, Liver targeting | CRISPR-Cas9 delivery, tumor therapy, animal model | Chen X. et al. (2020) |
| AR@PSS@PCM (ANP) |
|
1,064 | — | Photothermal activation, deep tissues | PD-L1 targeting, ICD activation | CRISPR-Cas9 delivery, improving immune checkpoint blockade, animal model | Tang et al. (2021) |
| Protamine–AuNCs |
|
700–1,000 | — | NIR-I imaging, deep tissues | E7, U2OS, HeLa cells | CRISPR-Cas9 delivery, tumor therapy, in vivo | Tao et al. (2021) |
| Photolabile semiconducting polymer nanotransducer |
|
680/808 | 700–1,000 | Photothermal activation, deep tissues | Plk1, Hela cells | CRISPR-Cas9 delivery, animal model | Lyu et al. (2019) |
| NTA-SS-PEG-PCL/Ce6 Complex |
|
1,064 | 700–1,000 | Photothermal conversion, deep tissues | Nrf2, CNE-2 cells | CRISPR-Cas9 delivery, tumor therapy, animal model | Deng et al. (2020) |
| UCNPs-Cas9@PEI |
|
980 | — | Photothermal activation, upconversion, deep tissues | Plk1, kB cells, A549 cells | CRISPR-Cas9 delivery, tumor therapy, animal model | Pan et al. (2019) |
| Upconversion nanoparticles |
|
700–1,000 | — | Photothermal activation, deep tissues | DNA 5-cytosine methylation, HEK293T cells | CRISPR-Cas9 delivery, animal model | Chi et al. (2021) |
| Gold nanorod (AuNR) |
|
808 | 700–1,000 | Photothermal therapy, deep tissues | EGFP, EMX1, A549, HEK293T cells | Gene editing, in vivo | Peng et al. (2020) |
| NIR light-triggered thermo-responsive copper sulfide (CuS) |
|
808 | — | Photothermal therapy, deep tissues | Hsp90α, A375 cells | CRISPR-Cas9 delivery, tumor therapy, animal model | Chen et al. (2021) |
| Si-rhodamine 680SiR |
|
680 | 700 | NIR-I imaging, deep tissues | SNAP targeting, tubulin2 targeting, U2OS cells | Dual-color confocal and STED imaging, in vivo | Butkevich et al. (2018) |