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. 2021 Dec 16;12:792465. doi: 10.3389/fimmu.2021.792465

Figure 4.

Figure 4

Treatment with CD52 antibody decreases inflammatory activation in the spinal cord of C57BL/6J EAE mice. C57BL/6J EAE mice were treated with anti-CD52 antibodies at the peak of disease. Four and 14 days after treatments, the spinal cord was isolated from EAE mice for the analysis of Iba1-positive cell infiltration and inflammatory gene transcription. Treatment with CD52 antibodies decreased the number of Iba1-positive cells (in brown) in the white matter of anterior and lateral columns at the lumber spinal cord compared with PBS-treated EAE mice 4 and 14 days after treatments (A–C) t test; t (14) = 2.608 and t (12) = 3.951 for 4 and 14 days, respectively; n ≥ 6 per group; The images are from EAE mice 14 days post treatments). Similarly, treatments with anti-CD52 antibodies strongly decreased the transcription of both pro- and anti-inflammatory genes (D–J) t test; t (11) = 4.903, 3.181, 4.176, 2.681, 5.120, 2.739 and 2.233 for tnf-α, il-1β, ccl-2, cxcl-10, il-10, ifn-γ and il-17a, respectively; n ≥ 5 per group).