TABLE 1.
Characteristics of S. aureus strains analyzed
| Straina | Date of isolation (mo/yr) | Origin (infection [I] or colonization [C]) | PFGE pattern | Phage typeb | MIC (μg/ml)
|
|||
|---|---|---|---|---|---|---|---|---|
| VAN
|
TEC
|
|||||||
| Agar dilution method with MH agar/BHI agar | E-test | Agar dilution method with MH agar/BHI agar | E-test | |||||
| Glycopeptide-susceptible strains | ||||||||
| ATCC 25923c | B | NDd | 2/2 | 2 | 2/2 | 2 | ||
| RA48c | 01/99 | Blood (I) | C | III | 1/1 | 2 | 1/2 | 2 |
| DE49e | 08/98 | Wound (C) | D | NTf | 1/2 | 3 | 1/2 | 3 |
| HE50e | 11/98 | PBSg (I) | E | ND | 1/1 | 2 | 1/2 | 2 |
| HO51e | 12/98 | Urine (I) | E (2)h | ND | 1/2 | 3 | 1/2 | 2 |
| MB52e | 01/99 | Wound (I) | F | IV | 1/1 | 2 | 1/1 | 2 |
| LO53e | 01/99 | Wound (C) | G | V | 1/1 | 2 | 1/1 | 2 |
| SE54e | 01/99 | Wound (C) | ND | NT | 2/2 | 2 | 1/2 | 2 |
| BA55e | 01/99 | Wound (C) | H | ND | 1/1 | 2 | 1/2 | 2 |
| ST56e | 02/99 | Urine (I) | H | ND | ND | 3 | ND | 2 |
| CR57e | 03/99 | PBS (I) | E | ND | ND | 2 | ND | 1.5 |
| CH58e | 03/99 | PBS (I) | E | VI | ND | 2 | ND | 2 |
| Strains with decreased susceptibility to glycopeptidese | ||||||||
| 46063 | 11/92 | Blood (I) | A | II | 2/4 | 4 | 8/8 | 12 |
| PE41 | 10/96 | Blood (I) | A | I | 4/4 | 6 | 4/8 | 12 |
| KE42 | 06/97 | Wound (C) | A | I | 4/4 | 4 | 4/8 | 8 |
| TR43 | 07/97 | PBS (I) | A (1) | I | 4/4 | 4 | 4/8 | 12 |
| BE44 | 08/97 | Wound (C) | A | I | 4/4 | 6 | 4/8 | 16 |
| RI45 | 09/97 | Wound (C) | A | I | 4/4 | 4 | 4/8 | 8 |
| MO01 | 10/98 | Blood (I) | A | I | 2/4 | 4 | 4/8 | 12 |
| CA02 | 10/98 | Blood (I) | A | I | 4/4 | 4 | 4/8 | 8 |
| PE03 | 11/98 | Drainage fluid (I) | A | I | 2/4 | 4 | 4/8 | 12 |
| ER04 | 11/98 | Sputum (I) | A | I | 4/4 | 4 | 4/8 | 12 |
| MA05 | 11/98 | PBS (I) | A | NT | 2/4 | 4 | 4/8 | 12 |
| BE06 | 11/98 | Wound (I) | A | I | 4/4 | 6 | 4/8 | 12 |
| LA07 | 11/98 | Wound (C) | A | I | 4/4 | 4 | 4/8 | 12 |
| DA08 | 11/98 | Wound (C) | A | I | 4/4 | 6 | 4/8 | 12 |
| BO09 | 11/98 | Nose (C) | A | I | 4/4 | 6 | 4/8 | 12 |
| GN10 | 11/98 | PBS (C) | A | I | 4/4 | 6 | 4/8 | 12 |
| HA11 | 11/98 | Wound (C) | A | NT | 4/4 | 4 | 4/4 | 8 |
| HE12 | 11/98 | PBS (I) | A | I | 4/4 | 4 | 4/8 | 16 |
| SM13 | 12/98 | Wound (C) | A | I | 4/4 | 4 | 4/8 | 12 |
| RI14 | 12/98 | PBS (C) | A | NT | 4/4 | 4 | 4/8 | 12 |
| BE15 | 12/98 | Catheter (I) | A | I | 4/4 | 4 | 4/8 | 12 |
| ZE16 | 12/98 | Nose (C) | A | I | 4/4 | 4 | 4/8 | 16 |
| DE17 | 12/98 | PBS (I) | A | I | 4/4 | 6 | 4/8 | 12 |
| DE18 | 12/98 | Wound (I) | A (4) | I | 4/4 | 8 | 16/32 | 32 |
| TE19 | 12/98 | Wound (C) | A | I | 4/4 | 4 | 4/8 | 12 |
| SI20 | 12/98 | Catheter (I) | A | I | 4/4 | 6 | 4/8 | 12 |
| RA21 | 12/98 | Wound (C) | A | I | 4/4 | 4 | 4/8 | 12 |
| GA22 | 12/98 | Sputum (C) | A | I | 4/4 | 6 | 4/8 | 12 |
| LA23 | 01/99 | Catheter (C) | A | I | 4/4 | 6 | 4/8 | 12 |
| TU24 | 01/99 | PBS (C) | A | NT | 4/4 | 4 | 4/4 | 12 |
| HE25 | 01/99 | Electrode (I) | A | I | 4/4 | 6 | 4/8 | 12 |
| PI26 | 01/99 | PBS (I) | A | NT | 4/4 | 6 | 4/8 | 12 |
| FA27 | 01/99 | Wound (C) | A | I | 4/4 | 4 | 4/8 | 12 |
| LE28 | 01/99 | Wound (C) | A | I | 4/4 | 6 | 4/8 | 16 |
| ES29 | 01/99 | Sputum (C) | A | I | 4/4 | 6 | 4/8 | 16 |
| VE30 | 02/99 | Wound (C) | A | NT | 4/4 | 4 | 4/8 | 12 |
| PO31 | 02/99 | Wound (C) | A | I | 4/4 | 4 | 4/8 | 12 |
| AU32 | 02/99 | Urine (I) | A | I | 4/4 | 4 | 4/8 | 12 |
| OB33 | 02/99 | Urine (I) | A | I | 4/4 | 4 | 4/8 | 12 |
| KA34 | 02/99 | Pleural catheter (C) | A | ND | 4/4 | 4 | 4/8 | 12 |
| JO35 | 02/99 | Catheter (I) | A | ND | 4/4 | 6 | 4/8 | 16 |
| LA36 | 02/99 | Nose (C) | A (4) | ND | 4/4 | 8 | 4/8 | 16 |
| GA37 | 03/99 | Blood (I) | A | ND | 4/4 | 6 | 4/8 | 16 |
| CA38 | 03/99 | Wound (I) | A | ND | 4/4 | 6 | 4/8 | 12 |
| HA39 | 03/99 | Nose (C) | A (1) | ND | 4/4 | 4 | 4/8 | 8 |
| TE40 | 03/99 | Rectal (C) | A | ND | 4/4 | 4 | 4/8 | 12 |
a
All strains except ATCC 25923 and strain 46063 were isolated at Broussais Hospital. Strain ATCC 25923 was from the American Type Culture Collection, and strain 46063 was isolated at Saint Joseph Hospital (9).
b
Phage type designations were arbitrarily assigned (I through VI).
c
Methicillin susceptible.
d
ND, not determined.
e
Methicillin resistant.
f
NT, not typeable.
g
PBS, protected brush specimen.
h
The values in parentheses are the number of bands that differ with respect to the predominant pattern.