FIG. 10.

Rapamycin treatment diminishes insulin-stimulated GLUT4 translocation in 3T3-L1 adipocytes. 3T3-L1 adipocytes expressing the GLUT4 reporter were cultured in DMEM with the indicated concentrations of rapamycin for 36 h and starved for the final 12 h of this period. Cells were then stimulated with 160 nM insulin for the indicated amounts of time, chilled, stained to detect externalized Myc epitope, and subjected to FACS to determine the relative proportion of GLUT4 at the cell surface in each sample. In the presence of increasing concentrations of rapamycin, GLUT4 was translocated to progressively fewer degrees by insulin stimulation.