Fig. 3.

Cresyl violet stained sections hippocampus of control (A-E), 40 min (F-J), and 60 min (K-O) exposed mice formed by Cornu Ammonis (CA) as CA1, CA2 & CA3 areas. It continued as a subiculum (S). Around the upper and lower limbs of CA3, the dentate gyrus (DG) is located. In control mice, the star indicates healthy neurons. The nuclei of cells and Nissl granular constituents of the cytoplasm appeared dark violet in all four regions in control mice (B-E). The rest of the cytoplasm appeared lightly stained. In radiation-exposed mice, the arrowheads represent degenerated neurons with dark cytoplasm and shrinkage in the cell body. In 40 min, exposed mouse pyramidal cells in CA1, CA2, CA3 regions (G, H, I) and granule cells (J) in the DG of the showed degenerated with darkly stained nuclei (arrowhead). In CA1, CA2, CA3 regions (L, M, N) and granular layer of the DG (O) of 60 min exposed mice showed the increased number of disorganized degenerated with darkly stained shrunken morphology pyramidal neuron cells (arrowhead) compared to the control and 40 min mice. Scale bar = 100 μm in A, F, K, and 20 μm in B-E, G-J, and L-O. (n = 10 mice in each group).