Fig. 5.

Agar diffusion assay: The antimicrobial activity of biogenic AgNPs against Streptococcus pneumonia and Staphylococcus aureus bacterial strain is showed in the form of zone inhibition on a solid nutrient agar plate. The highest zone of inhibition against Streptococcus pneumonia and Staphylococcus aureus were 18 and 24 mm, respectively. The agar plate A represents the Streptococcus pneumonia untreated, whereas; the plate F represents untreated Staphylococcus aureus. Panel B to E represents the bacterial strain Streptococcus pneumonia being treated with AgNPs at a concentration of 50 µg/ml. In contrast, Panel G to J represents Staphylococcus aureus at 50 µg/ml. The zone of inhibition assay has been performed in a quadruplicate to achieve the best standard results. All the experiments were performed in triplicate and represented as ± SEM.