TABLE 3.
Correlation between reactivity in the immunoblot and quantitative CP5 expression measured by ELISA inhibition
| Expt no. and S. aureus strain | Source | Immunoblot reactivitya | Amt (μg) of CP5/1010 CFU | % of control |
|---|---|---|---|---|
| 1 | ||||
| Reynolds (control) | Human | 4+ | 518 | 100 |
| PS15rr (U.S.) | Bovine | 4+ | 518 | 100 |
| AA405 (U.S.) | Bovine | 4+ | 411 | 79 |
| Myco 15 (U.S.) | Bovine | 4+ | 278 | 54 |
| 2 | ||||
| Reynolds (control) | Human | 4+ | 2,400 | 100 |
| PS Milk (U.S.) | Bovine | 2–3+ | 209 | 8.7 |
| Gutz 5 (U.S.) | Bovine | 2+ | 91 | 3.8 |
| 3263 (U.S.) | Bovine | 2+ | 42 | 1.8 |
| KK 13 (U.S.) | Bovine | 1+ | 52 | 2.2 |
| 3 | ||||
| Reynolds (control) | Human | 4+ | 1,984 | 100 |
| AA26 (U.S.) | Bovine | 2–3+ | 120 | 6.0 |
| WSU 1 (U.S.) | Bovine | 2+ | 64 | 3.2 |
| Myco 11 (U.S.) | Bovine | 2+ | 13 | 0.7 |
| Hook 14 (U.S.) | Bovine | 1–2+ | 13 | 0.7 |
| 4 | ||||
| 7125-2 (control) (Sweden) | Bovine | 4+ | 1,177 | 100 |
| 6698-1 (Sweden) | Bovine | ± | 4 | 0.3 |
| 55-46 (Finland) | Bovine | ± | 4 | 0.3 |
| 78-9 (Finland) | Bovine | ± | 16 | 1.4 |
| 2076 (Iceland) | Bovine | ± | 6 | 0.5 |
a
1+, very weak; 2+, medium; 3+, strong; 4+, very strong; ±, little to none.