Fig. 1.

ELISA of Noggin in the supernatant.

(A) Schematics of the experiment. Cells were either exposed to (black) or not given (white) BMP4 and the supernatant was harvested for ELISA at day 1, 2, and 3 (arrows). (B) Immunostained cells cultured with (left) and without (right) BMP4 conditions at day 3. SOX2 (green): undifferentiated marker. Brachyury (red): mesoderm marker. DAPI (blue): nuclei. Initial cell densities were 1 × 10⁴ and 5 × 10⁴ cells/cm2. (C) Noggin ELISA results. Four Noggin concentrations in independent supernatants were collected from different cell cultures at different passages. The vertical axis is Noggin concentration in the supernatant. (Mean ± S.E., n = 4). The horizontal dotted line indicates the mean of all the data (0.69 ng/mL). (For interpretation of the references to colour in this figure legend, the reader is referred to the Web version of this article.)