Fig. 2.

Simulation of the simple static culture.

(A) Scheme of the simulation of static culture. The cells are placed at the bottom (y = 0), which is covered with the culture medium (y > 0). Each square is 20 μm by 20 μm. (B) Scatter plot of the spatially averaged [N(y)] concentration in the medium (y > 0) and total exposed BMP4 at t = 72 h when the barrier-like structure was not applied under each condition. The open circles and closed rectangles represent 10 and 50 ng/mL BMP4 concentrations, respectively. Vertical dotted line indicates the threshold of cell differentiation (100 h ng/mL) and the right of the line correspond to differentiated cells (gray). Horizontal dotted line indicates the measured level of Noggin (0.69 ng/mL). The open and closed arrow are the crossing points between the undifferentiated and differentiated area.