Table 2.
The impact of hypoxia on the cytostatic effect of various prostate cancer cell lines was assessed by crystal violet growth assay as in Fig. 1 and described in Materials and Methods. All cell lines were pre-treated for 24 h with 1% O2 or 21% O2 before the addition of various doses of YM155 or vehicle control and cells were cultured in their respective growth conditions for an additional 72 h before crystal violet staining. The IC50 values were assessed by dose-response curves using GraphPad Prism software and represent the average of triplicate biological determinations ± SE.
| Cell Line | IC50 (nM)Normoxic | IC50 (nM)Hypoxic | Fold Change |
|---|---|---|---|
| PC-3 | 1.82 ± 0.14 | 26.8 ± 2.6 | 14.9 |
| DU-145 | 3.95 ± 0.11 | 58.9 ± 2.0 | 15 |
| C4-2 | 8.7 ± 0.6 | 163.9 ± 18.7 | 19.4 |
| LNCaP | 44 ± 5.4 | >300 | >6.8 |
| 22RV1 | 8.15 ± 0.43 | 202.5 ± 14.8 | 24.8 |