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. 2021 Dec 11;3:100061. doi: 10.1016/j.crtox.2021.12.001

Fig. 1.

Fig. 1

No significant toxicity effects are induced in HEK293T cells by exposure to Pb. A. Cell survival was assessed by MTT assay. The results represent the means of 3 biological experiments with six technical repeats. No significant (p > 0.05, one-way ANOVA followed by Tukey’s HSD post-hoc test) changes were observed at selected Pb doses, 100 and 500 ppb. B. Growth curves of HEK293T after treating with Pb, 100 and 500 ppb. Pb treatment slow down cell growth, but no significant (p > 0.05, one-way ANOVA followed by Tukey’s HSD post-hoc test) effect was found in the doubling time (15.2 h, 15.7 h and 16.7 h for 0, 100, and 500 ppb of Pb, respectively). The arrow indicates the time when Pb was added to the cell culture. Data is presented as the mean of 3 biological repeats. C. Exposure to Pb revealed no significant (p > 0.05, one-way ANOVA followed by Tukey’s HSD post-hoc test) modification in the nuclear size or eccentricity of HEK293T cells. Data presented correspond to at least 25 cells analyzed per biological repeat, N = 3.