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. 2021 Dec 11;3:100061. doi: 10.1016/j.crtox.2021.12.001

Fig. 3.

Fig. 3

meCpG levels in HEK293T cells exposed to Pb. A. Representative 2D projected images of HEK293T cells after 24 h of transfection with meCpG probes and pre-exposed for 24 h to Pb, 100 or 500 ppb. Scale bar = 2 µm. B. Relative meCpG levels to the control or 0 ppb of Pb using the integrated intensity per nuclei and integrated foci intensity per nuclei obtained from image analysis. Data is presented as the mean ± S.D., N = 3. C. Probability density distribution of the integrated intensity per nuclei and integrated foci intensity per nuclei obtained from single cell image analysis, n ≥ 80. Presented data corresponds to at least 25 cells analyzed per biological repeat, N = 3. D. Expression of mRNAs coding for DNMT1 and TET1. Data is expressed as cycle threshold (Ct) difference to β-actin presented as dot plots with indicated mean value. Data is presented as the mean ± S.D., N = 3. N.S.: stands for no significant difference. * represents p < 0.05 from a one-way ANOVA followed by Tukey’s HSD post hoc test.