Figure 8.

RPLP1 and RPLP2 require regulatory HBV sequences to function on HBsAg production

(A) Schematics of the HBV sequences cloned in plasmid pcDNA3.1 tested for HBsAg production. The nucleotide number of the HBV genome is shown at the flanks of the DNA sequence. The green arrow represents the human cytomegalovirus promoter. The green box labeled with “An” represents the polyadenylation sequence. Both the promoter and polyadenylation sequence are present in pcDNA3.1. Boxes represent the coding sequence for HBV genes. The two red ovals denote HBV enhancer sequences. (B) Relative amounts of the HBsAg produced in HEK 293 cells transfected with STOPS, siRNAs, or ALG-20002. HBsAg levels were reproducibly increased by the knockdown of GRP78 in cells transfected with pPol_SAgtercDNA3.1 and pSAg-PsicDNA3.1. The percentages of HBsAg expressed from these plasmids relative to the mock-treated control (pCDNA3.1) are shown above the blue bars. Each error bar represents one standard deviation of uncertainty.