Official websites use .gov
A
.gov website belongs to an official
government organization in the United States.
Secure .gov websites use HTTPS
A lock (
) or https:// means you've safely
connected to the .gov website. Share sensitive
information only on official, secure websites.
Schematic of IRS-PCR analysis. (Step 1) Double digestion using EagI and HhaI. (Step 2) Adapter ligation. (Step 3) Primary denaturation followed by EagI primer binding. (Step 4) If the selective primer extension is a cytosine, then Taq polymerase reads through and initial extension occurs. If X ≠ C, extension does not occur. (Step 5) Initial extension produces complementary binding sequence for oligonucleotide AH1 (from HhaI adapter).
