Figure 3.

Negative depletion of WBCs with elastomeric particles. (A) WBC depletion efficiency and (B) WBC fold depletion after the two-step acoustophoresis (A²) of cancer cells with or without Pluronic in the buffer. (C) Cancer cell (DU145) recovery after A² with or without Pluronic in the buffer. The graphs show three experiments (n = 3) with blood from three different healthy donors. (D) ImageStream DU145 cell stained with anti-EpCAM-APC (I), one WBC stained with DAPI bound to an elastomeric particle (II). Two DAPI stained WBCs bound to the same elastomeric particle (III). One DAPI stained WBC bound to three elastomeric particles (IV).