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Charging and gas-phase stabilities of WT and supercharged GFP variants. (A) Coulombic surface potentials predicted for the three GFP variants. Structural models were generated using Rosetta; the His6 tag is omitted for clarity. (B) Mass spectra of GFPAc, GFPWT, and GFPBas show near-identical CSDs dominated by the 9+ and 10+ charge states. (C) CIU profiles of the 9+ charge state of each variant indicate that GFPBas has a higher resistance to unfolding than GFPWT and GFPAc.
