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Design and feasibility of the redox regeneration pathway. (A) Scheme of the reactions and their coupling for generation of NADH, NADPH, and GSH. (B) Purity of the enzymes after Ni-Sepharose and size-exclusion chromatography (SEC). The SEC profile was monitored at 280 nm. On the top right corner of each frame, we show the sodium dodecyl sulfate (SDS)-polyacrylamide gel of the corresponding protein peak.
