. 2021 Nov 12;1(12):2280–2293. doi: 10.1021/jacsau.1c00406

Table 1. Overview of the Pathway Enzymes and Their Properties.

	systematic name	EC number	organism	molecular weight (kDa) (native mass)	oligomeric state	substrates	K_M (mM)	k_CAT (s^–1)	refs
Fdh	formate:NAD⁺ oxidoreductase	1.17.1.9	S. novella	93	dimer	NAD⁺	0.11	1.08	this study
Fdh	formate:NAD⁺ oxidoreductase	1.17.1.9	S. novella	93	dimer	formate	2.15	0.87	this study
SthA	NADPH:NAD⁺ oxidoreductase	1.6.1.1	E. coli	432	octamer	NADH	2.63	9.7	this study
SthA	NADPH:NAD⁺ oxidoreductase	1.6.1.1	E. coli	432	octamer	(thio)NADP⁺	0.03	19.9	this study
GorA	glutathione:NADP⁺ oxidoreductase	1.8.1.7	E. coli	102	dimer	GSSG	0.07	733.3	(27, 28)
GorA	glutathione:NADP⁺ oxidoreductase	1.8.1.7	E. coli	102	dimer	NADPH	0.02	661.8	(27, 28)