FIG. 2.

Subcellular localization of CDC25A and ASK1. Human embryonic kidney 293 cells, transfected with pcDNA3 expression vectors for CDC25A and HA-tagged ASK1, were stained with anti-CDC25A monoclonal antibody and anti-HA polyclonal antibody (upper panels). Human ovarian carcinoma OVCAR-8 cells, without transfection, were stained with anti-CDC25A monoclonal antibody and anti-ASK1 polyclonal antibody (lower panels). Signals were visualized by incubation with fluorescein-conjugated antimouse IgG antibody and Texas red-conjugated anti-rabbit IgG antibody, followed by analysis with a confocal laser-scanning microscope. These results are representative of three independent sets of experiments. Bar, 5 μm.