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. 2021 Dec 11;25(1):103586. doi: 10.1016/j.isci.2021.103586

Figure 3.

Figure 3

Single-molecule imaging of target-foundation colocalization

(A) Single-molecule images of RC coupled with Atto 647 for three different FC:RC ratios 1:0.5, 1:1, and 1:3. Imaging exposure time was 500 ms with a laser output of 7 mW. Scale bars are 10 μm.

(B) Single-molecule imaging demonstrating colocalization of FC:RC at ratio 1:1. Scale bars are 10 μm.

(C) Microscopy reveals efficient co-localization of barcode foundations with peptide targets on the BCS chip. Colocalization efficiency at the different cololinker ratios where 1:1 possessed the highest efficiency. Two experiments were performed and five tiles each were analyzed. Error bars represent standard error. A ratio of 1:1 (FC:RC) was selected for BCS experiments to ensure the highest possible number of ligated targets had an associated foundation.