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. 2021 Aug 23;70(1):29–35. doi: 10.1136/jim-2020-001715

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© American Federation for Medical Research 2022. Re-use permitted under CC BY-NC. No commercial re-use. Published by BMJ.

This is an open access article distributed in accordance with the Creative Commons Attribution Non Commercial (CC BY-NC 4.0) license, which permits others to distribute, remix, adapt, build upon this work non-commercially, and license their derivative works on different terms, provided the original work is properly cited, an indication of whether changes were made, and the use is non-commercial. See: http://creativecommons.org/licenses/by-nc/4.0/.

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Effect of butyrate on renal tubular epithelial cell apoptosis and cell proliferation after renal ischemia/reperfusion (I/R). (A) Terminal deoxynucleotidyl transferase-mediated dUTP-biotin nick-end labeling (TUNEL) assay on renal slices of sham-operated, saline and butyrate-treated rats. High TUNEL-positive renal tubular epithelial cell numbers were observed in saline-treated rats after I/R, whereas only very limited TUNEL-positive cells were observed in butyrate-treated rats. Representative images of TUNEL-positive staining cells. (B) In saline-treated group, the number of positive cells were higher than in butyrate pretreatment group. Data were shown as means±SD, n=10/group. *P<0.05 vs the sham group, #p<0.05 vs the vehicle group.