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Evaluation of identifications in DDA and DIA-TMT acquisition strategies. Comparison of (A) DDA and (B) DIA experiments, showing the numbers of MS/MS scans (black), PSMs (brown), and unique peptides (blue) using (C) SpectroMine database search (DDA) or (D) Spectronaut library search (DIA) at various peptide input levels (i.e., 0.5 ng, 1 ng, 5 ng, 10 ng). Mean and standard deviations are shown.
