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. 2021 Dec 24;3(1):101072. doi: 10.1016/j.xpro.2021.101072

PCR cycling conditions

Steps Temperature Time Cycles
Initial Denaturation 98°C 30 s 1
Denaturation 98°C 10 s 30 cycles
Annealing 57°C 30 s
Extension 72°C 1 min
Final extension 72°C 10 min 1
Hold 4°C 2min