REAGENT or RESOURCE	SOURCE	IDENTIFIER
Chemicals, peptides, and recombinant proteins
Oatmeal Agar	BD	Cat#DF0552-17-3
Sucrose	Fisher Scientific	Cat#S5-12
Yeast extract	BD	Cat#21750
Casamino acid	VWR	Cat#J851-500G
Yeast Nitrogen Base Without Amino Acids and Ammonium Sulfate	Fisher Scientific	Cat#DF0335-15-9
Ammonium nitrate	Sigma-Aldrich	Cat#221244-500G
L- (+) - Asparagine	Fisher Scientific	Cat#AAB2147322
Glucose	Sigma-Aldrich	Cat#G8270-100G
Agarose, low gelling temperature	Sigma-Aldrich	Cat#A9414-5G
di-sodium hydrogen phosphate	Sigma-Aldrich	Cat#1065851000
Bacteriological Agar	IBI Scientific	Cat#IB49171
Hygromycin B in solution	Corning	Cat#45000-806
G418 solution	VWR	Cat#97064-358
Bialaphos/glufosinate ammonium	Crescent Chemical	Cat#C140300
FK506	LC Laboratories	Cat#NC0876958
Agarose	VWR	Cat#0710-500G
LbCas12a protein	New England BioLabs	Cat#M0653T
Lysing Enzymes from Trichoderma harzianum	Sigma-Aldrich	Cat#L1412-10G
Sodium chloride	Fisher Scientific	Cat#S271-3
PEG4000	FLUKA	Cat#81240
Potassium chloride	Sigma-Aldrich	Cat#P-4504
Chloroform	Sigma-Aldrich	Cat#C2432-1L
Isopropanol	Fisher Scientific	Cat#A416-4
Ethanol	Fisher Scientific	Cat#BP2818-500
Tris-HCI, pH 8.0	Thermo Fisher Scientific	Cat#AM9856
Calcium chloride	Sigma-Aldrich	Cat#C8106-1KG
GeneRuler 1 kb Plus DNA Ladder	Thermo Fisher Scientific	Cat#SM1331
Critical commercial assays
Phusion® High-Fidelity DNA Polymerase	New England BioLabs	Cat#M0530L
Q5® High-Fidelity DNA Polymerases	New England BioLabs	Cat#M0491L
Taq DNA Polymerase with Standard Taq Buffer	New England BioLabs	Cat#M0273L
HiScribe™ T7 High Yield RNA Synthesis Kit	New England BioLabs	Cat#E2040S
Monarch® RNA Cleanup Kit	New England BioLabs	Cat#T2050L
DNase I (RNase-free)	New England BioLabs	Cat#M0303S
Deoxynucleotide (dNTP) Solution Mix	New England BioLabs	Cat#N0447L
Wizard SV Gel and PCR Clean-Up system	Promega	Cat#A9282
Experimental models:Organisms/strains
M. oryzae field isolate O-137	Dr. Barbara Valent (Kansas State Univ)	N/A
Oligonucleotides
Please see the oligos used in this protocol in Table S1	Huang et al. (2021)	N/A
Recombinant DNA
pFGL821	Addgene	Cat#58223
pFGL921	Zhang et al. (2021)	N/A
pBV9/pSM324	Kankanala et al. (2007)	N/A
Software and algorithms
BLAST	FungiDB	https://fungidb.org/fungidb/app/search/transcript/UnifiedBlast
Other
Membrane Filter, 0.22 μm pore size	Millex	Cat#SLGVM33RS
Plastic syringes	Thermo Fisher Scientific	Cat#S7510-10
Toothpick	Fisher Scientific	Cat#S24554
Hemocytometer	Fisher Scientific	Cat#0267151B
Miracloth	Calbiochem	Cat#475855-1R
61/2 in Disks Non Gauze Milk Filter	KenAG	N/A
Water, Molecular Biology Grade	VWR	Cat#VWRL0201-0500
150 × 15 mm petri dishes	Fisher Scientific	Cat#FB0875714
60 × 15 mm Petri dishes	MIDSCI	Cat#901
100 × 15 mm Petri dishes	MIDSCI	Cat#900
50 mL falcon tubes	MIDSCI	Cat#C50B
1.5 mL microcentrifuge tubes	USA scientific	Cat#1615-5500
0.2 mL PCR tubes	MIDSCI	Cat#AVTW-F
Funnels	Fisher Scientific	Cat#FB6015865
125 mL flask	PYREX	N/A
200 mL flask	PYREX	N/A
Parafilm	Sigma-Aldrich	Cat#P7793-1EA
NanoDrop spectrophotometer	Thermo Fisher Scientific	N/A
2.0 mL impact resistant screw cap tube	USA scientific	Cat #1420-9600
1.0 mm Silica Beads	BioSpec	Cat #11079110z
Bead ruptor elite-Bead mill homogenizer	OMNI international	Cat#19-2141E
Centrifuge	Eppendorf	Cat#5415D
Centrifuge	Beckman Coulter	Cat#Allegra 25R
Filter paper	N/A	N/A