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TB3 medium (TB3)
| Reagent | Final concentration | Amount |
|---|---|---|
| Sucrose | 200 g/L | 200 g |
| Yeast extract | 6 g/L | 6 g |
| Casamino acid | 6 g/L | 6 g |
| Agar | 15 g/L | 15 g |
| ddH2O | n/a | up to 1000 mL |
| Total | n/a | 1000 mL |
Autoclave at 121°C for 20 min in liquid cycle. Agar is not required for liquid TB3.
Store at room temperature up to 6 months.
