Protoplast lysing solution

Reagent	Final concentration	Amount
Lysing Enzymes from Trichoderma harzianum	10 mg/mL	0.3 g
0.7M NaCl solution	n/a	up to 30 mL
Total	n/a	30 mL

Filter sterilize using 0.22 μm filter. This solution needs to be freshly prepared on the date when you are ready to perform protoplast preparation.