Alignment of NEPH1 with homologous proteins. The human
NEPH1 (hNEPH1; GenBank accession no. AYO17369), a truncated mouse NEPH1
(mNEPH1; GenBank accession no. AYO17368), the D.
melanogaster irregular chiasmata (IRREGULAR; GenBank accession
no. L11040), and the human NEPHRIN (hNEPHRIN; GenBank accession no.
AF190637) proteins were aligned using the MultiAlin version 5.4.1
program (http://www.toulouse.inra.ft/multalin). The
signal and transmembrane (TM) domains in the hNEPH1, mNEPH1, and
IRREGULAR proteins, identified by DNAStar Protean V. 4.0, are
underlined. Ig-like domains (Ig) are marked by broken lines, and the
PKD domain is doubly underlined; these domains were identified using
the Pfam search engine (http://pfam.wustl.edu). The RGD
sequence of NEPH1 is marked by asterisks. The hNEPHRIN protein sequence
extends an additional 459 amino acid residues beyond what is shown
here.