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. 2021 Dec 16;9:773050. doi: 10.3389/fcell.2021.773050

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Copyright © 2021 Shi, Han, Yao, Liu, Jiang and Yan.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Characterization of endothelial progenitor cells (EPCs). (A) Staining of Dil-conjugated acetylated low-density lipoprotein (Dil-Ac-LDL), FITC-Ulex Europaeus Agglutinin I (UEA-1), and merged images of double staining of Dil-Ac-LDL and FITC-UEA-I. Scale bar: 20 μm. (B) Immunofluorescent staining demonstrated that EPCs had a positive expression of CD133, CD34, and VEGFR2. Scale bar: 20 μm. (C) Flow cytometry analysis showed the expressions of CD133, CD34, and VEGFR2 in EPCs. All experiment was repeated three times.