Fig. 6.

FTH rescues the impaired mitochondrial homeostasis upon ferroptosis. A Control or FTH overexpression HCCLM3 cells were treated with cystine-free medium, then stained by mitoTracker-Red and JC-10. The morphological changes of mitochondria and JC-10 fluorescence intensities were observed under confocal microscope (Scale bars 10 μm). B Control and FTH ectopic expression cells were treated with cystine-free medium for 8 h, the mitochondrial oxygen consumption rate (OCR) was monitored by Seahorse analyzer. C–F The OCR values of maximal respiration, ATP production, spare respiratory capacity, and non-mitochondrial respiratory were calculated. (Values are represented as mean ± SD. ^★P < 0.05, ^★★P < 0.01 versus indicated groups)