Table 2.
NDH-1 Deamino-NADH oxidasea | NDH-2 NADH oxidaseb | |||
---|---|---|---|---|
Inhibitor | IC50 | Maximum inhibition | IC50 | Maximum inhibition |
(μM)c | (%)c | (μM)c | (%)c | |
TF1 | 45 | 95 | 3.0 | 75 |
TF2A | 15 | 95 | 3.5 | 95 |
TF2B | 8 | 95 | 2.5 | 90 |
TF3 | 7 | 90 | 1.5 | 95 |
EGCG | 20 | 90 | 20 | 65 |
NDH-1 activity using deamino-NADH was about 0.5 μmoles deamino-NADH/min/mg protein
NDH-2 activity using NADH was about 0.25 μmoles NADH/min/mg protein
IC50 concentrations and maximum inhibition % were determined from two replicates of 5-6 concentrations of inhibitor. The data are displayed in Supplementary Figure2.