. Author manuscript; available in PMC: 2021 Dec 30.

Published in final edited form as: J Nutr Biochem. 2011 Sep 15;23(8):953–960. doi: 10.1016/j.jnutbio.2011.05.001

Table 2.

Inhibition of NADH Oxidase Activity by Theaflavins

	NDH-1 Deamino-NADH oxidase^a		NDH-2 NADH oxidase^b
Inhibitor	IC₅₀	Maximum inhibition	IC₅₀	Maximum inhibition
Inhibitor	(μM)^c	(%)^c	(μM)^c	(%)^c
TF1	45	95	3.0	75
TF2A	15	95	3.5	95
TF2B	8	95	2.5	90
TF3	7	90	1.5	95
EGCG	20	90	20	65

NDH-1 activity using deamino-NADH was about 0.5 μmoles deamino-NADH/min/mg protein

NDH-2 activity using NADH was about 0.25 μmoles NADH/min/mg protein

IC₅₀ concentrations and maximum inhibition % were determined from two replicates of 5-6 concentrations of inhibitor. The data are displayed in Supplementary Figure2.