Table 3:
Markers of injury in the lung and liver
| Surfactant Additive | Controls | Saline | Bud 0.25 | Bud 0.10 | Bud 0.04 |
|---|---|---|---|---|---|
| Protective VT ventilation (n=5–6/group) | |||||
| Lung IL-1β mRNA | 1±0.6 | 11±7 | 6±7 | 9±5 | 21±20 |
| Lung IL-6 mRNA | 1±0.6 | 75±26 | 22±18 * | 55±55 | 151±144 |
| Lung MCP-1 mRNA | 1±0.3 | 99±93 | 15±13 * | 31±33 | 74±67 |
| Lung ENAC mRNA | 1±0.1 | 20±11 | 66±24 * | 45±20 | 42±30 |
| Lung Injury Score (out of 8) | 0.4±0.3 | 4.5±2.5 | 3.7±2.0 | 2.0±0.7 | 3.0±1.7 |
| Lung iNOS+ cells (out of 2) | 0±0 | 0.7±0.8 | 0.3±0.8 | 0.1±0.2 | 0.3±0.4 |
| Liver MCP-1 mRNA | 1±0.3 | 7±7 | 0.5±0.4 * | 0.6±0.6 * | 3±3 |
| Liver SAA3 mRNA | 1±1 | 20±7 | 52±52 | 22±19 | 29±18 |
| Injurious Ventilation for first 15 minutes (n=5–8/group) | |||||
| Lung IL-1β mRNA | 1±0.6 | 104±112 | 10±15t | 16±29 | 11±11 |
| Lung IL-6 mRNA | 1±0.6 | 2117±2876 | 59±89t | 87±75 | 183±182 |
| Lung MCP-1 mRNA | 1±0.3 | 376±347 | 39±23t | 74±60 | 72±57 |
| Lung ENAC mRNA | 1±0.1 | 20±9 | 37±19t | 34±11t | 41±20t |
| Lung Injury Score (out of 8) | 0.4±0.3 | 6.3±1.6 | 4.4±1.5t | 5.0±1.1 | 5.9±2.6 |
| Lung iNOS+ cells (out of 2) | 0±0 | 1.2±0.7 | 0.5±0.6t | 0.5±0.5 | 0.9±1.2 |
| Liver MCP-1 mRNA | 1±0.3 | 79±103 | 9±21t | 11±11 | 7±7 |
| Liver SAA3 mRNA | 1±1 | 79±103 | 66±43 | 26±12t | 37±30 |
mRNA data represents fold change compared to unventilated controls. All surviving animals to 6 hours were analyzed. Mean±SD
*
p < 0.05 vs Protective VT + Saline, t = p < 0.05 vs Injury VT + Saline