Fig. 3.

Biocompatibility of parylene-coated 3D prints. A) Schematic cross-sectional representation of a 96-well plate with the 3D printed insert used for biocompatibility testing. B) Representative images of iPSC-derived neurons labeled with calcein (live cells) and propidium iodide (dead cells) after 24 and 48 hours of soluble contact with the 3D prints. C) Quantification of iPSC-derived neuron viability at different time points (percent of live cells versus total cells). Each well was imaged in 3 different locations, and 4 independent wells were imaged per condition representing the individual data points. On day 14, a one-way ANOVA was used to calculate statistical significance between the coated and uncoated conditions (p < 0.0001). D) Representative immunofluorescence images of the iPSC-derived neurons at day 21 after incubation with coated and uncoated 3D prints (grey resin). Inset highlights morphological differences.