FIG. 2.

Multiple determinants of cytosolic localization in c-Rel–IκBα protein complex. GFP-c-Rel and derivatives thereof were transiently transfected with HA-IκBα (first row) or its derivative nuclear export mutant NESn or NESnc as described in the legend to Fig. 1. GFP-c-Rel(RHD) contains the first 297 residues of c-Rel that include the RHD and the NLS (residues 292 to 295). GFP-c-Rel(344) contains the first 344 residues of c-Rel. Thirty-six hours after transfection, cells were immunostained for fluorescent visualization. Results shown are representative of at least three independent experiments.