FIG. 6.

c-Rel shuttles only in mature B cells. A20, D5h3, and 70Z cells were settled on poly-l-lysine-coated coverslips overnight. Half of the coverslips with attached cells were treated with LMB (100 ng/ml) for 45 min. Cells with and without LMB treatment were fixed and permeabilized for immunostaining with anti-p65 antibodies (A) or anti-c-Rel antibodies (B). Green fluorescence in the first and the third columns shows endogenous p65 (A) and endogenous c-Rel (B) of A20 (top row), 70Z (second row), and D5h3 (third row) cells before and after LMB treatment as indicated. Blue fluorescence in the second and fourth columns shows DAPI staining of nuclei. Results shown are from one of three independent experiments.