| Microscope |
Talos Arctica |
Talos Arctica |
| Detector (Mode) |
Falcon 3EC, counting mode |
Falcon 3EC, counting mode |
| Voltage (kV) |
200 |
200 |
| Magnification (nominal) |
92,000X |
120,000X |
| Total electron fluence (e−/Å2) |
36.35 |
44.34 |
| Electron flux (e−/pixel/sec) |
0.76 |
0.85 |
| Defocus range (µm) |
−1 – −1.75 |
−0.7 – −1.3 |
| Pixel size (Å) |
1.12 |
0.8757 |
| Total exposure time (sec) |
60 |
40 |
| Total fractions/micrograph |
45 |
62 |
| Exposure per fraction (e−/Å2/fraction) |
0.81 |
0.72 |
| Micrographs collected (no.) |
5,109 |
5,309 |
| Total extracted particles (no.) |
3,500,000 |
3,336,981 |
| Refined particles (no.) |
2,346,578 |
450,747 |
| Final particles (no.) |
110,433 |
112,170 |
| Symmetry imposed |
C1 |
C1 |
| Global Resolution (Å) |
| FSC 0.5 (masked/unmasked) |
4.4/6.5 |
4.6/8.3 |
| FSC 0.143 (masked/unmasked) |
3.9/4.3 |
4.2/5.2 |
| Local resolution range (Å) |
3.5 – 5.5 |
3.9 – 6.0 |
| Map Sharpening B factors (Å2) |
−45.12 |
−116.219 |
|
Model composition
|
| Non-hydrogen atoms |
27,399 |
12,727 |
| Protein residues |
3,483 |
1,854 |
| Ligands |
6 Mg2+, 2 ATP, 4 ADP, 5 Phalloidin |
Mg2+, 2 ATP |
|
Refinement
|
| R.m.s. deviations |
| Bond lengths (Å) |
0.004 |
0.004 |
| Bond angles (°) |
0.967 |
0.983 |
|
|
|
|
|
Validation
|
| EMRinger score |
1.39 |
1.29 |
| MolProbity score |
1.65 |
1.42 |
| Clashscore |
5.67 |
4.66 |
| Poor rotamers (%) |
0.00 |
0.00 |
| Cβ deviations (%) |
0.00 |
0.00 |
| Ramachandran plot |
| Favored (%) |
95.00 |
96.96 |
| Allowed (%) |
5.00 |
3.04 |
| Disallowed (%) |
0.00 |
0.00 |
| CaBLAM outliers (%) |
4.2 |
3.8 |
